期刊
JOURNAL OF BIOPHOTONICS
卷 11, 期 7, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201700225
关键词
bacteria detection; laser ablation in liquids; Raman spectroscopy; SERS; ultrapure laser-synthesized Au nanoparticles
资金
- Academy of Finland [1292253, 260321, 290596, 284907]
- Center for Research Strategy of Free University of Berlin [0503121810]
- Government of Russian Federation [074-U01]
- ITMO Plan Cancer 2014-2019 INSERM program [PC201420]
- COST project [ECOST-STSM-BM1205-120416-072252]
- European Cooperation in Science and Technology [BM1205-120416-072252]
- Academy of Finland (AKA) [284907, 284907] Funding Source: Academy of Finland (AKA)
The ability of noble metal-based nanoparticles (NPs) (Au, Ag) to drastically enhance Raman scattering from molecules placed near metal surface, termed as surface-enhanced Raman scattering (SERS), is widely used for identification of trace amounts of biological materials in biomedical, food safety and security applications. However, conventional NPs synthesized by colloidal chemistry are typically contaminated by nonbiocompatible by-products (surfactants, anions), which can have negative impacts on many live objects under examination (cells, bacteria) and thus decrease the precision of bioidentification. In this article, we explore novel ultrapure laser-synthesized Au-based nanomaterials, including Au NPs and AuSi hybrid nanostructures, as mobile SERS probes in tasks of bacteria detection. We show that these Au-based nanomaterials can efficiently enhance Raman signals from model R6G molecules, while the enhancement factor depends on the content of Au in NP composition. Profiting from the observed enhancement and purity of laser-synthesized nanomaterials, we demonstrate successful identification of 2 types of bacteria (Listeria innocua and Escherichia coli). The obtained results promise less disturbing studies of biological systems based on good biocompatibility of contamination-free laser-synthesized nanomaterials.
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