期刊
JOURNAL OF BIOMOLECULAR NMR
卷 70, 期 4, 页码 245-259出版社
SPRINGER
DOI: 10.1007/s10858-018-0183-4
关键词
MILVA labeling; 4D HMQC-NOESY-HMQC; Human blood group A and B glycosyltransferases; Methyl-TROSY; Paramagnetic relaxation enhancement
资金
- German Research Council DFG [Pe494/11-1]
- iNEXT Program [PID 1483, PID 2254]
Human blood group A and B glycosyltransferases (GTA, GTB) are highly homologous glycosyltransferases. A number of high-resolution crystal structures is available showing that these enzymes convert from an open conformation into a catalytically active closed conformation upon substrate binding. However, the mechanism of glycosyltransfer is still under debate, and the precise nature as well as the time scales of conformational transitions are unknown. NMR offers a variety of experiments to shine more light on these unresolved questions. Therefore, in a first step we have assigned all methyl resonance signals in MILVA labeled samples of GTA and GTB, still a challenging task for 70 kDa homodimeric proteins. Assignments were obtained from methyl-methyl NOESY experiments, and from measurements of lanthanide-induced pseudocontact shifts (PCS) using high resolution crystal structures as templates. PCSs and chemical shift perturbations, induced by substrate analogue binding, suggest that the fully closed state is not adopted in the presence of lanthanide ions.
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