期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 23, 期 4, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.23.4.046005
关键词
biomedical optics; molecular imaging; surgical guidance; Raman spectroscopy; nanomedicine
资金
- NIH/NIBIB [R21 EB015016]
- NIH/NCI [R21 CA215561]
- Department of Mechanical Engineering at the University of Washington
- Department of Education GAANN fellowship program
- NIH [P30-CA015704]
- Northwest BioTrust (NWBT)
Surface-enhanced Raman scattering (SERS) nanoparticles (NPs) are increasingly being engineered for a variety of disease-detection and treatment applications. For example, we have previously developed a fiber-optic Raman-encoded molecular imaging (REMI) system for spectral imaging of biomarker-targeted SERS NPs topically applied on tissue surfaces to identify residual tumors at surgical margins. Although accurate tumor detection was achieved, the commercial SERS NPs used in our previous studies lacked the signal strength to enable high-speed imaging with high pixel counts (large fields of view and/or high spatial resolution), which limits their use for certain time-constrained clinical applications. As a solution, we explored the use of surface-enhanced resonant Raman scattering (SERRS) NPs to enhance imaging speeds. The SERRS NPs were synthesized de novo, and then conjugated to HER2 antibodies to achieve high binding affinity, as validated by flow cytometry. Under identical tissue-staining and imaging conditions, the targeted SERRS NPs enabled reliable identification of HER2-overexpressed tumor xenografts with 50-fold-enhanced imaging speed compared with our standard targeted SERS NPs. This enables our REMI system to image tissue surfaces at a rate of 150 cm(2) per minute at a spatial resolution of 0.5 mm. (C) 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据