期刊
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
卷 14, 期 1, 页码 198-205出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2018.2524
关键词
Loop-Mediated Isothermal Amplification; Turbidimeter; Legionella; H7N9; Optical Detector
资金
- NSFC [61527806, 61471168]
- Hunan Key Research Project [2017SK2174]
- China Postdoctoral Science Foundation [2016T90403]
- Economical Forest Cultivation and Utilization of Collaborative Innovation Center in Hunan Province [(2013) 448]
- Scientific Research Project of Hunan Education Department [17C0486]
This study aimed at developing a portable multi-channel turbidity system (21 cm in length, 15.5 cm in width and 11.5 cm in depth) by real-time loop-mediated isothermal amplification (LAMP) method for rapid detection of pathogens. The developed system herein includes temperature control unit, photoelectric detection unit, turbidity calibration unit, power management unit, human machine unit, communication unit and ARM-based microcontroller. The coefficient of variation for eight channels is less than 0.25% in noise analysis. Legionella bacteria (LEG) and H7 subtype virus (H7) were successively detected by the designed and developed system within 60 minutes. Moreover, its specificity for LEG is satisfactory and its sensitivity for H7 is 10 copies/mL. Besides, this system for point-of-care diagnosis allows a rapid, small size, low cost, and automatic detection with the characteristics of high-efficiency, excellent stability and high uniformity.
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