期刊
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
卷 55, 期 10, 页码 -出版社
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.14-15047
关键词
sclera; mouse; collagen; X-ray scattering; glaucoma
资金
- Fight for Sight Project [1360]
- Medical Research Council Program [MR/K000837/1]
- National Institutes of Health [EY02120, EY01765, EY021500]
- Science and Technology Facilities Council
- MRC [MR/K000837/1] Funding Source: UKRI
- Fight for Sight [1359/60] Funding Source: researchfish
- Medical Research Council [MR/K000837/1] Funding Source: researchfish
PURPOSE. The organization of scleral collagen helps to determine the eye's biomechanical response to intraocular pressure (IOP), and may therefore be important in glaucoma. This study provides a quantitative assessment of changes in scleral collagen fibril organization in bead-induced murine experimental glaucoma. METHODS. Wide-angle X-ray scattering was used to study the effect of bead-induced glaucoma on posterior scleral collagen organization in one eye of 12 CD1 mice, with untreated fellow eyes serving as controls. Three collagen parameters were measured: the local preferred fibril directions, the degree of collagen anisotropy, and the total fibrillar collagen content. RESULTS. The mouse sclera featured a largely circumferential orientation of fibrillar collagen with respect to the optic nerve head canal. Localized alteration to fibril orientations was evident in the inferior peripapillary sclera of bead-treated eyes. Collagen anisotropy was significantly (P < 0.05) reduced in bead-treated eyes in the superior peripapillary (Treated: 43 +/- 8%; Control: 49 +/- 6%) and midposterior (Treated: 39 +/- 4%; Control: 43 6 4%) sclera, and in the peripapillary region overall (Treated: 43 +/- 6%; Control: 47 +/- 3%). No significant differences in total collagen content were found between groups. CONCLUSIONS. Spatial changes in collagen fibril anisotropy occur in the posterior sclera of mice with bead-induced chronic IOP elevation and axonal damage. These results support the idea that dynamic changes in scleral form and structure play a role in the development of experimental glaucoma in mice, and potentially in human glaucoma.
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