期刊
JOURNAL OF BIOMECHANICS
卷 73, 期 -, 页码 99-107出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jbiomech.2018.03.040
关键词
Proteomics; Labor; Phosphorylation; Preterm; Uterus
资金
- Mountain West Clinical Translational Research-Infrastructure Network under a grant from the National Institute of General Medical Sciences (NIH) [1U54GM104944]
- National Institute of General Medical Sciences from the National Institutes of Health [8 P20 GM103440]
- Pathway to Independence Award from the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NIH) [R00HD067342]
Mechanical strain associated with the expanding uterus correlates with increased preterm birth rates. Mechanical signals result in a cascading network of protein phosphorylation events. These signals direct cellular activities and may lead to changes in contractile phenotype and calcium signaling. In this study, the complete phospho-proteome of uterine smooth muscle cells subjected to mechanical strain for 5 min was compared to un-strained controls. Statistically significant, differential phosphorylation events were annotated by Ingenuity Pathway Analysis to elucidate mechanically induced phosphorylation networks. Mechanical strain leads to the direct activation of ERK1/2, HSPB1, and MYL9, in addition to phosphorylation of PAK2, vimentin, DOCK1, PPP1R12A, and PTPN11 at previously unannotated sites. These results suggest a novel network reaction to mechanical strain and reveal proteins that participate in the activation of contractile mechanisms leading to preterm labor. (C) 2018 Elsevier Ltd. All rights reserved.
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