期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 293, 期 18, 页码 6824-6843出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.RA117.001074
关键词
DNA replication; DNA polymerase; human; crystal structure; DNA-protein interaction; RNA; conformational change; chimeric RNA-DNA primer; Pol; Pol; Pol epsilon; pre-steady-state kinetics
资金
- NCI, National Institutes of Health (NIH), Cancer Center Support Grant [P30CA036727]
- NIGMS, NIH [P41 GM103403, INBRE-P20GM103427-14, COBRE-1P30GM110768-01]
- Department of Energy [DE-AC02-06CH11357]
- Fred and Pamela Buffett Cancer Center Support Grant [P30CA036727]
- NATIONAL CANCER INSTITUTE [P30CA036727] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P41GM103403, P30GM110768, P20GM103427, R01GM049551, R35GM127085, R01GM101167, T32GM067543] Funding Source: NIH RePORTER
DNA polymerase (Pol) plays an important role in genome replication. In a complex with primase, Pol synthesizes chimeric RNA-DNA primers necessary for replication of both chromosomal DNA strands. During RNA primer extension with deoxyribonucleotides, Pol needs to use double-stranded helical substrates having different structures. Here, we provide a detailed structure-function analysis of human Pol's interaction with dNTPs and DNA templates primed with RNA, chimeric RNA-DNA, or DNA. We report the crystal structures of two ternary complexes of the Pol catalytic domain containing dCTP, a DNA template, and either a DNA or an RNA primer. Unexpectedly, in the ternary complex with a DNA:DNA duplex and dCTP, the fingers subdomain of Pol is in the open conformation. Pol induces conformational changes in the DNA and hybrid duplexes to produce the universal double helix form. Pre-steady-state kinetic studies indicated for both duplex types that chemical catalysis rather than product release is the rate-limiting step. Moreover, human Pol extended DNA primers with higher efficiency but lower processivity than it did with RNA and chimeric primers. Pol has a substantial propensity to make errors during DNA synthesis, and we observed that its fidelity depends on the type of sugar at the primer 3-end. A detailed structural comparison of Pol with other replicative DNA polymerases disclosed common features and some differences, which may reflect the specialization of each polymerase in genome replication.
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