期刊
ANALYTICAL LETTERS
卷 47, 期 16, 页码 2761-2773出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/00032719.2014.917422
关键词
Brombuterol; Chemiluminescent enzyme immunoassay; Clenbuterol; Polyclonal antibody; Salbutamol; Urine
资金
- Special Fund for Agro-Scientific Research in the Public Interest [201203069-2]
- Fund of Modern Agriculture Industry System Innovation in Beijing City Team
A salbutamol-bovine serum albumin and a salbutamol-ovalbumin coating antigen were synthesized, and six New Zealand white rabbits were treated with the immunogen to obtain polyclonal antibodies to develop a rapid, sensitive, and indirect chemiluminescent enzyme immunoassay (ciCLEIA) for the analysis of swine and bovine urine. The prepared antibodies showed high cross-reactivities with clenbuterol (139.6%) and brombuterol (225%). Under the optimized conditions, the linear dynamic range and the limit of detection (LOD) for salbutamol were from 0.007 to 0.17 mu g L-1 and 0.003 mu g L-1, with a correlation coefficient of 0.9965 and a half maximum inhibition concentration of 0.028 mu g L-1. Recoveries for salbutamol, clenbuterol, and brombuterol were from 78.8% to 119.0% with intra-assay and inter-assay coefficients of variation less than 13.9% and 19.7%, respectively. The reported ciCLEIA was about 10-fold more sensitive for salbutamol and 20-fold more sensitive for clenbuterol compared to conventional methods. This study showed that ciCLEIA was a reliable, convenient, and sensitive method for the simultaneous determination of salbutamol, clenbuterol, and brombuterol in swine and bovine urine.
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