期刊
GENOME
卷 58, 期 2, 页码 71-79出版社
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/gen-2015-0002
关键词
amphidiploid; Triticum aestivum; wheat; Aegilops; Secale; Thinopyrum; Triticum; chromosome doubling; genomic in situ hybridization
资金
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/100260X/1]
- University of Nottingham
- BBSRC [BB/I00260X/1, BB/K021141/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/I00260X/1, BB/K021141/1] Funding Source: researchfish
We aim to improve diversity of domesticated wheat by transferring genetic variation for important target traits from related wild and cultivated grass species. The present study describes the development of F-1 hybrids between wheat and related species from the genera Aegilops, Secale, Thinopyrum, and Triticum and production of new amphidiploids. Amphidiploid lines were produced from 20 different distant relatives. Both colchicine and caffeine were successfully used to double the chromosome numbers. The genomic constitution of the newly formed amphidiploids derived from seven distant relatives was determined using genomic in situ hybridization (GISH). Altogether, 42 different plants were analysed, 19 using multicolour GISH separating the chromosomes from the A, B, and D genomes of wheat, as well as the distant relative, and 23 using single colour GISH. Restructuring of the allopolyploid genome, both chromosome losses and aneuploidy, was detected in all the genomes contained by the amphidiploids. From the observed chromosome numbers there is an indication that in amphidiploids the B genome of wheat suffers chromosome losses less frequently than the other wheat genomes. Phenotyping to realize the full potential of the wheat - related grass germplasm is underway, linking the analyzed genotypes to agronomically important target traits.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据