期刊
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
卷 73, 期 7, 页码 1812-1814出版社
OXFORD UNIV PRESS
DOI: 10.1093/jac/dky105
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资金
- Robert Koch Institute
- German Ministry of Health [1369-402]
Background: Antimicrobial resistance due to carbapenemase expression poses a worldwide threat in health-care. Inter-genus exchange of genetic information is of utmost importance in this context. Objectives: Here, to the best of our knowledge, we describe the first detection and characterization of a KPC-2-producing Pseudomonas aeruginosa in Germany. Methods: Characterization of the isolate was performed using MALDI-TOF MS, automated microdilution and MLST. Carbapenemase detection was performed using phenotypic and genotypic assays. The bla(KPC-2)-carrying plasmid was transformed into Escherichia coli NEB VR 10-beta. The purified plasmid DNA was sequenced using the Illumina technique. Results: The isolate expressed ST235 and was resistant to carbapenems. Antimicrobial susceptibility testing revealed colistin to be the only antimicrobial agent active in vitro. The bla(KPC-2) gene was located on a replicon type lncHI1 plasmid as part of Tn4401. Conclusions: The first detection (to the best of our knowledge) of plasmid-encoded KPC-2 in P. aeruginosa in Germany may point to a currently underestimated spread of carbapenemases among clinically relevant Gram-negative bacteria. Here, to the best of our knowledge, we also provide the first report of bla(KPC-2) associated with the IncHI1 plasmid.
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