期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 141, 期 1, 页码 269-+出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2017.02.039
关键词
Vitamin D; asthma; innate immunity; pregnancy
资金
- National Institute for Health Research (NIHR) Clinical Research Facility at Guy's & St Thomas' NHS Foundation Trust
- NIHR Biomedical Research Centre based at Guy's and St Thomas' NHS Foundation Trust
- King's College London
- Asthma UK [08/019, MRC-Asthma UK Centre, MRC-AsthmaUKCentre] Funding Source: researchfish
- Medical Research Council [G1000758B, G1000758] Funding Source: researchfish
Background: Programming of the immune system during fetal development can influence asthma-related risk factors and outcomes in later life. Vitamin D is a well-recognized immune modulator, and deficiency of this nutrient during pregnancy is hypothesized to influence disease development in offspring. Objective: We sought to investigate the effect on neonatal immunity of maternal supplementation with 4400 IU/d vitamin D-3 during the second and third trimesters of pregnancy by using a subset of cord blood samples from a randomized, double-blind, placebo-controlled clinical trial (the Vitamin D Antenatal Asthma Reduction Trial). Methods: Cord blood samples from neonates born to mothers supplemented with 4400 IU/d (n=26) or 400 IU/d (n=25) of vitamin D-3 were analyzed for immune cell composition by flow cytometry, Toll-like receptor (TLR) expression by quantitative PCR, and cytokine secretion after stimulation with mitogenic, TLR, and T-cell stimuli by cytometric bead array. Responsiveness to the glucocorticoid dexamethasone was determined. Results: Supplementation of mothers with 4400 IU of vitamin D-3 resulted in an enhanced broad-spectrum proinflammatory cytokine response of cord blood mononuclear cells to innate and mitogenic stimuli (P=.0009), with an average 1.7- to 2.1-fold increase in levels of several proinflammatory cytokines (GMCSF, IFN-gamma, IL-1 beta, IL-6, and IL-8) across stimuli, a higher gene expression level of TLR2 (P=.02) and TLR9 (P=.02), a greater than 4-fold increase in IL-17A (P=.03) production after polyclonal T-cell stimulation, and an enhanced IL-10 response of cord blood mononuclear cells to dexamethasone treatment in culture (P=.018). Conclusion: Vitamin D exposure during fetal development influences the immune system of the neonate, which can contribute to protection from asthma-related, including infectious, outcomes in early life.
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