期刊
LEARNING & MEMORY
卷 21, 期 11, 页码 616-626出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/lm.035972.114
关键词
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资金
- Belgian Queen Elisabeth Medical Foundation
- Belgian Fund for Scientific Research (FRS-FNRS)
- Medical Research Council [G0800393] Funding Source: researchfish
- MRC [G0800393] Funding Source: UKRI
alpha-calcium/calmodulin-dependent protein kinase (alpha CaMKII) T286-autophosphorylation provides a short-term molecular memory that was thought to be required for LTP and for learning and memory. However, it has been shown that learning can occur in alpha CaMKII-T286A mutant mice after a massed training protocol. This raises the question of whether there might be a form of LTP in these mice that can occur without T286 autophosphorylation. In this study, we confirmed that in CA1 pyramidal cells, LTP induced in acute hippocampal slices, after a recovery period in an interface chamber, is strictly dependent on postsynaptic alpha CaMKII autophosphorylation. However, we demonstrated that alpha CaMKII-autophosphorylation-independent plasticity can occur in the hippocampus but at the expense of synaptic specificity. This nonspecific LTP was observed in mutant and wild-type mice after a recovery period in a submersion chamber and was independent of NMDA receptors. Moreover, when slices prepared from mutant mice were preincubated during 2 h with rapamycin, high-frequency trains induced a synapse-specific LTP which was added to the nonspecific LTP. This specific LTP was related to an increase in the duration and the amplitude of NMDA receptor-mediated response induced by rapamycin.
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