期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 66, 期 20, 页码 5178-5189出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b01620
关键词
rapeseed antioxidant peptide; absorption of intestinal epithelial cells; bioavailability; in vivo metabolism mechanism; inhibition of HUVEC apoptosis
资金
- National Key Research and Development Program of China [2016YFD0400201, 2016YFD0401401]
- National Natural Science Foundation of China [31571766]
- Science and Technology Project of Nanjing [201716034]
- Prospective Industry-Academy-Research Cooperation Projects of Jiangsu Province [BY2016009-01]
- National Agricultural Achievements Transformation Projects [2014GB2C100318]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
WDHHAPQLR (RAP) is an antioxidative peptide derived from rapeseed protein. Although the health benefits from RAP, due to its antioxidant activities, have been determined by chemical methods, a systematic assessment regarding the absorption, metabolism, and antioxidation processes of RAP is still lacking attention. Hence, Caco-2 cell monolayer models and animal experiments were used to evaluate the absorption and bioavailability of RAP. As expected, RAP could be absorbed by intestinal epithelial cells, and the Papp was 0.82 +/- 0.19 X 10(-6) cm/s. Three main fragments, RAP, DHHAPQLR, and WDHHAP were transported by the paracellular pathway, and QLR was transported by PepT1. An important modified product of RAP (EGDHHAPQLR) was found to contribute to the elimination of intracellular reactive oxygen species. The absolute bioavailability of RAP was 3.56%, and three degradation products of RAP were also detected in rat serum. More importantly, RAP exerts its antioxidant activity by inhibiting the apoptosis of oxidative stress cells. RAP could downregulate the expression of Bax and caspase-3 and upregulate the expression of Bcl-2 in H2O2-induced HUVECs (human umbilical vein endothelial cells). In general, using in vitro and in vivo experimental models, the in vivo absorption and transformation processes of RAP and its antioxidative molecular mechanisms by inhibiting apoptosis of cells were revealed.
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