期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 66, 期 19, 页码 4872-4882出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b00960
关键词
macro algae; laver; bioactive peptides; alpha-amylase inhibition; diabetes mellitus
资金
- National Natural Science Foundation of China [31522044]
- Fundamental Research Funds for the Central Universities [JUSRP51406A]
Inhibition of alpha-amylase enzyme is one therapeutic approach in lowering glucose level in the blood to manage diabetes mellitus. In this study alpha-amylase inhibitory peptides were identified from proteolytic enzymes hydrolysates of red seaweed laver (Porphyra species) using consecutive chromatographic techniques. In the resultant fractions from RP-HPLC (D-1-(10)), D-2 inhibited alpha-amylase activity (88.67 +/- 1.05%) significantly (p <= 0.5) at 1 mg/mL protein concentration. A mass spectrometry (ESI-Q-TOF- MS) analysis was used to identify peptides from this fraction. Two novel peptides were identified as Gly-Gly-Ser-Lys and Glu-Leu-Ser. To validate their alpha-amylase inhibitory activity, these peptides were synthesized chemically. The peptides were demonstrated inhibitory activity at IC50 value: 2.58 +/- 0 08 mM (Gly-Gly-Ser-Lys) and 2.62 +/- 0.05 mM (Glu-Leu-Ser) The inhibitory kinetics revealed that these peptides exhibited noncompetitive binding mode. Thus, laver can be a potential source of novel ingredients in food and pharmaceuticals in diabetes mellitus management.
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