期刊
GENETICS AND MOLECULAR RESEARCH
卷 14, 期 3, 页码 9306-9317出版社
FUNPEC-EDITORA
DOI: 10.4238/2015.August.10.11
关键词
Bambusa oldhamii; Gene cloning; Sequence analysis; TERMINAL FLOWER1; Transgenic Arabidopsis thaliana
资金
- Ministry of Science and Technology of China [2012CB723008]
- Top Key Discipline of Forestry of Zhejiang Province [KF201304]
- National the Natural Science Foundation of China [31270715, 31000295]
TERMINAL FLOWER1 (TFL1) homologous genes play major roles in maintaining vegetative growth and inflorescence meristem characteristics in various plant species; however, to date, the function of the bamboo TFL1 homologous gene has not been described. In this study, a TFL1 homologous gene was isolated from Bambusa oldhamii and designated as BoTFL1-like. Phylogenetic analysis of TFL1 homologous genes revealed that BoTFL1-like shared more than 90% identity with the TFL1 genes of other Gramineae. RT-PCR analysis showed that the expression level of BoTFL1-like in floral buds was almost 3.5 times higher than in vegetative buds. In 35S:: BoTFL1-like transgenic Arabidopsis thaliana plants, the time of flowering was significantly delayed by 5 to 9 days, and development of floral buds and sepals was severely affected compared to wild type Arabidopsis plants. This suggests that the BoTFL1-like gene may play roles in flowering time and flower morphological structure in B. oldhamii. The BoTFL1-like gene driven by the 35S promoter almost fully rescued the phenotype of the tfl1 mutant apart from the number of rosette inflorescences, indicating that the function of BoTFL1-like was similar to TFL1 in Arabidopsis. We conclude the TFL1 gene function has been conserved between B. oldhamii and A. thaliana.
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