期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 66, 期 8, 页码 1949-1954出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.7b05326
关键词
lateral flow test strip; aptamer; zearalenone; gold nanoparticles
资金
- China Postdoctoral Science Foundation [2015M580402, 2016T90430]
- Key Research and Development Program of Jiangsu Province [BE2016306]
- Natural Science Foundation of Jiangsu Province [BK20140155]
An aptamer-based lateral flow test strip was developed for the detection of zearalenone (ZEN). This assay was based on the competition for the aptamer between ZEN and its complementary sequence. Several experimental conditions that could influence sensitivity have been investigated, including the concentration of aptamer and NaCl used in the probe preparation, the mole ratio of streptavidin and biotinylated DNA used in the preparation of test line and control line, and the loading quantity of gold nanoparticles-aptamer conjugates (AuNPs-Apt). Under the optimal experimental conditions, we successfully detected ZEN within a detection range of 5-200 ng/mL and the visual limit of detection of 20 ng/mL. This aptamer-based strip was successfully applied to the determination of ZEN in spiked corn samples, and the recoveries were from 93.4% to 114.2%. All detections can be achieved within 5 min. The results demonstrated that the developed aptamer-based lateral flow test strip is a potential alternative tool for the rapid and sensitive detection of ZEN.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据