期刊
GENETICS AND MOLECULAR RESEARCH
卷 14, 期 4, 页码 13667-13678出版社
FUNPEC-EDITORA
DOI: 10.4238/2015.October.28.29
关键词
Agrobacterium tumefaciens; Drought stress; Oryza officinalis; Gene transformation; Wild rice
资金
- Guangdong Provincial Key Natural Science Foundation [10251064201000001, 2014A030311042]
- Guangdong provincial key platform of University and major research project (Natural Science) - characteristic innovation project [Yue-JK2014 (65)]
- Special Fund for Agro-Scientific Research in the Public Interest [201003021]
- Guangdong science and technology program [2014A030304055]
Oryza officinalis has proven to be a natural gene reservoir for the improvement of domesticated rice as it carries many desirable traits; however, the transfer of elite genes to cultivated rice by conventional hybridization has been a challenge for rice breeders. In this study, the conserved sequence of plant stress-related NAC transcription factors was selected as a probe to screen the O. officinalis genomic transformation-competent artificial chromosome library by Southern blot; 11 positive transformation-competent artificial chromosome clones were subsequently detected. By Agrobacterium-mediated transformation, an id/ca rice variety, Huajingxian 74 (HJX74), was transformed with a TAO clone harboring a NAC gene-positive genomic fragment from O. officinalis. Molecular analysis revealed that the O. officinalis genomic fragment was integrated into the genome of HJX74. The transgenic lines exhibited high tolerance to drought stress. Our results demonstrate that the introduction of stress-related transformation-competent artificial chromosome clones, coupled with a transgenic validation approach, is an effective method of transferring agronomically important genes from O. officinalis to cultivated rice.
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