期刊
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
卷 55, 期 10, 页码 6272-6279出版社
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
DOI: 10.1167/iovs.14-14648
关键词
refractive development; myopia; rod photoreceptors; dopamine
资金
- National Institutes of Health (NIH) [R01 EY016435, P30 EY006360, R01 EY004864]
- Department of Veterans Affairs Rehabilitation R&D Service Research Career Scientist Award
- Research to Prevent Blindness
PURPOSE. Proper refractive eye growth depends on several features of the visual image and requisite retinal pathways. In this study, we determined the contribution of rod pathways to normal refractive development and form deprivation (FD) myopia by testing Gnat1(-/-) mice, which lack functional rods due to a mutation in rod transducin-alpha. METHODS. Refractive development was measured in Gnat1(-/-) (n = 30- 36) and wild-type (WT) mice (n = 5-9) from 4 to 12 weeks of age. FD was induced monocularly from 4 weeks of age using head-mounted diffuser goggles (Gnat1(-/-), n = 9-10; WT, n = 7-8). Refractive state and ocular biometry were obtained weekly using a photorefractor, 1310 nm optical coherence tomography, and partial coherence interferometry. We measured retinal dopamine and its metabolite, DOPAC, using HPLC. RESULTS. During normal development, the refractions of WT mice started at 5.36 +/- 0.68 diopters (D) and became more hyperopic before plateauing at 7.78 +/- 0.64 D. In contrast, refractions in Gnat 1(-/-) mice were stable at 7.39 +/- 1.22 D across all ages. Three weeks of FD induced a 2.54 +/- 0.77 D myopic shift in WT mice, while Gnat 1(-/-) mice did not respond to FD at any age. Axial lengths of Gnat 1(-/-) and WT mice increased with age, but differences between genotypes or with goggling did not reach statistical significance and fell within the precision of the instruments. The DOPAC levels were significantly lower in Gnat 1(-/-) mice from 2 to 12 weeks of age with DOPAC/dopamine ratio peaking earlier in Gnat 1(-/-) compared to WT mice. No differences in dopamine were seen in response to FD or between genotypes. CONCLUSIONS. Functional rod photoreceptors are critical to normal refractive development and the response to FD in mice. Dopamine levels may not directly modulate the refractive state of the mouse eye, but tonic levels of dopamine during development may determine susceptibility to myopia.
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