期刊
GENETICS AND MOLECULAR RESEARCH
卷 14, 期 2, 页码 5896-5909出版社
FUNPEC-EDITORA
DOI: 10.4238/2015.June.1.7
关键词
Follicular development; Granulosa cell; Sprague-Dawley rat; Hypoxia-inducible factor-1 alpha; Proliferation cell nuclear antigen
资金
- National Natural Science Foundation of China [31101032, 31271255]
- Program for New Century Excellent Talents in University of Ministry of Education of China [NCET-120614]
- Doctoral Foundation of the Ministry of Education in China [20113503120002]
- Fujian Provincial Science and Technology Projects of the Department of Education [JB14041]
Hypoxia-inducible factor-1 alpha (HIF-1 alpha) has been identified as a transcription factor that is involved in diverse physiological and pathological processes in the ovary. In this study, we examined whether HIF-1 alpha is expressed in a cell-and stage-specific manner during follicular growth and development in the mammalian ovaries. Using immunohistochemistry and Western blot analysis, HIF-1 alpha expression was observed in granulosa cells specifically and was significantly increased during the follicular growth and development of postnatal rats. Furthermore, pregnant mare serum gonadotropin also induced HIF-1 alpha expression in granulosa cells and ovaries during the follicular development of immature rats primed with gonadotropin. Moreover, we also examined proliferation cell nuclear antigen, a cell proliferation marker, during follicular growth and development and found that its expression pattern was similar to that of HIF-1 alpha protein. Granulosa cell culture experiments revealed that proliferation cell nuclear antigen expression may be regulated by HIF-1 alpha. These results indicated that HIF-1 alpha plays an important role in the follicular growth and development of these 2 rat models. The HIF-1 alpha-mediated signaling pathway may be an important mechanism regulating follicular growth and development in mammalian ovaries in vivo.
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