期刊
ISME JOURNAL
卷 12, 期 8, 页码 1929-1939出版社
SPRINGERNATURE
DOI: 10.1038/s41396-018-0109-x
关键词
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资金
- Australian Research Council (ARC) [DP120100163, DP170104038]
- U.S. Department of Energy's Office of Biological Environmental Research [DE-SC0010574]
- University of Queensland International Scholarship
- China Scholarship Council Scholarship
- Australian Postgraduate Award
- University of Queensland Postdoctoral Fellowship
- ARC Discovery Early Career Researcher Award
- ARC
- University of Queensland
- University of Queensland Vice-Chancellor's Research Focused Fellowship
- Advanced Queensland Research Fellowship
- U.S. Department of Energy (DOE) [DE-SC0010574] Funding Source: U.S. Department of Energy (DOE)
Microbially mediated anaerobic oxidation of methane (AOM) is a key process in the regulation of methane emissions to the atmosphere. Iron can serve as an electron acceptor for AOM, and it has been suggested that Fe(III)-dependent AOM potentially comprises a major global methane sink. Although it has been proposed that anaerobic methanotrophic (ANME) archaea can facilitate this process, their active metabolic pathways have not been confirmed. Here we report the enrichment and characterisation of a novel archaeon in a laboratory-scale bioreactor fed with Fe(III) oxide (ferrihydrite) and methane. Long-term performance data, in conjunction with the C-13- and Fe-57-labelling batch experiments, demonstrated that AOM was coupled to Fe(III) reduction to Fe(II) in this bioreactor. Metagenomic analysis showed that this archaeon belongs to a novel genus within family Candidatus Methanoperedenaceae, and possesses genes encoding the reverse methanogenesis pathway, as well as multi-heme c-type cytochromes which are hypothesised to facilitate dissimilatory Fe(III) reduction. Metatranscriptomic analysis revealed upregulation of these genes, supporting that this archaeon can independently mediate AOM using Fe(III) as the terminal electron acceptor. We propose the name Candidatus Methanoperedens ferrireducens for this microorganism. The potential role of M. ferrireducens in linking the carbon and iron cycles in environments rich in methane and iron should be investigated in future research.
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