Marinicella sediminis sp nov., isolated from marine sediment

A novel heterotrophic, Gram-stain-negative, aerobic, rod-shaped, pale yellow, non-motile and non-spore-forming bacterium, designated strain F2(T), was isolated from marine sediment collected from the Weihai coast, Shandong Province, PR China. Optimal growth occurred at 33 degrees C (range, 10-37 degrees C), with 3.0-4.0 % (w/v) NaCl (1.0-8.0 %) and at pH 7.5-8.0 (pH 6.5-9.0). Q-8 was the sole respiratory quinone. The major polar lipids of strain F2(T) were phosphatidylmonomethylethanolamine, phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and two unidentified polar lipids. The major cellular fatty acid in strain F2(T) was iso-C-15:0. The genomic DNA G+C content of the strain was 48.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that strain F2(T) is most closely related to Marinicella litoralis JCM 16154(T) (97.5 %) and Marinicella pacifica sw153(T) (96.0 %). Based on the results of our polyphasic analysis, we conclude that strain F2(T) represents a novel species of the genus Marinicella, for which the name Marinicella sediminis sp. nov. is proposed. The type strain of the new species is F2(T) (=KCTC 42953(T)=MCCC 1H00149(T)).