Butyricicoccus porcorum sp nov., a butyrate-producing bacterium from swine intestinal tract

A Gram-stain-positive, non-motile, butyrate-producing coccus was cultured from the distal ileum of swine. This organism was isolated on rumen-fluid medium, consumes acetate, and produces butyrate as its major end product when grown on mono-and di-saccharides. A phylogenetic analysis based on near full-length 16S rRNA gene sequences as well as whole-genome phylogenies suggests that this isolate is most closely related to species in the genus Butyricicoccus, with Butyricicoccus pullicaecorum being the closest named relative (93.5% 16S similarity). The G+C content of this isolate is 54 mol %, and the major cellular fatty acids are C-18 : 0 DMA, C-14 : 0, C-18 : 1 omega 9c and C-16 : 0. These data indicate that this isolate represents a novel species within the genus Butyricicoccus, for which the name Butyricicoccus porcorum sp. nov. is proposed. The type strain of Butyricicoccus porcorum is BB10(T) (ATCC TSD-102 T, DSM 104997 T).