Pseudonocardia soli sp nov., isolated from mountain soil

A novel actinomycete, designated strain NW8-21(T), was isolated from mountain soil in Mae-Wong National Park, Nakornsawan province, Thailand, and was taxonomically characterized by using a polyphasic approach. Based on 16S rRNA gene sequence analysis, the strain was revealed to have the closest similarity to Pseudonocardia endophytica YIM 56035(T) with the highest 16S rRNA gene sequence similarity value of 98.7 %, followed by Pseudonocardia nantongensis KLBMP 1282(T) (98.0 %). The chemotaxonomic properties, i.e. arabinose and galactose as the diagnostic reducing sugar in cells, MK-8 (H-4) as a major menaquinone, liso-C-16:0 as the main cellular fatty acid component and phosphatidylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol, phosphatidylcholine as the characteristic phospholipids, confirmed a taxonomic affiliation of the strain that was consistent with those of the genus Pseudonocardia. Several phenotypic differences and the DNA-DNA hybridization results (less than 40% relatedness value) indicated that strain NW8-21(T) shoud be considered to represent a novel species of the genus Pseudonocardia, for which the name Pseudonocardia soli is proposed. The type strain is strain NW8-21(T) (= BCC 58125(T) = NBRC 109519(T)).