期刊
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
卷 94, 期 9, 页码 838-843出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09553002.2018.1492757
关键词
Biomphalaria glabrata; radiation; radiosensitization; usnic acid
资金
- Brazilian Fostering Agency CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
- State Fostering Agency FACEPE (Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco)
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
Purpose: Some phytochemicals have shown the potential of being radiomodifiers, especially phenolic compounds, such as lichenic secondary metabolites. To evaluate the phytochemical usnic acid as a radiomodifier, embryonic cells of molluscs have been used due to their ease of collection, high sensitivity to physical and chemical agents, well-known embryology and low cost for analysis. Materials and methods: This study aimed to assess the radiosensitizing action of usnic acid on Biomphalaria glabrata embryos. Samples were irradiated with 4 Gy of gamma rays from a Co-60 source (dose rate 2.906 Gy/h). An acute toxicity test was performed using B. glabrata embryos in the blastula stage, in order to determine the toxicity of usnic acid and to establish the lethal Concentration for 50% (LC50). Subsequently, the radiomodifing capacity of usnic acid was estimated using assays with B. glabrata embryos. Results: Irradiation increased the number of non-viable embryos compared to unirradiated controls. Additionally, it was observed that embryos exposed to a non-toxic concentration of usnic acid (0.6 mu g/mL) before irradiation showed a further enhancement in non-viable embryos when compared with exposure to ionizing radiation alone. Conclusion: The results presented here indicate that usnic acid makes cells more sensitive to the damaging effects of radiation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据