Nanosized titanium dioxide-induced premature ovarian failure is associated with abnormalities in serum parameters in female mice

Background: Exposure to titanium dioxide nanoparticles (TiO2 NPs) that are widely used in food, medicine, sunscreen products and cosmetics is reported to cause ovarian damage and lower fertility in animals. However, the potential effects of TiO2 NPs application on premature ovarian failure (POF) have rarely been evaluated to date. Methods: In this study, female mice were continuously exposed to TiO2 NPs at doses of 2.5, 5 or 10 mg/kg via gavage instillation for 30 days, and investigated the serum hormones and autoimmunity markers associated with POF. Results: Exposure to TiO2 NPs resulted in POF, reductions in the levels of estradiol, progesterone and inhibin B and increases in luteinizing hormone, follicle-stimulating hormone, folliclestimulating hormone/luteinizing hormone ratio, anti-Mullerian hormone, thyroid-stimulating hormone, free triiodothyronine, free tetraiodothyronine, anti-nuclear antibody and anti-thyroid peroxidase antibody levels in serum. Conclusion: Exposure to TiO2 NPs induced POF triggered by alterations in hormones and autoimmunity markers. Our findings highlight the necessity for significant caution in handling and usage of TiO2 NPs by female consumers.