期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 13, 期 -, 页码 2697-2708出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S151285
关键词
selenium nanoparticles; laser ablation in liquids; Candida albicans; biofilm; chitosan; synergy
资金
- National Institute on Minority Health and Health Disparities from the National Institutes of Health [G12MD007591]
- National Science Foundation Partnerships for Research and Education in Materials (NSF-PREM) [DMR-0934218]
- Welch Foundation [AX-1615]
- Biomedical Research Grants Program of the San Antonio Area Foundation
- Air Force Office of Scientific Research [FA9550-15-1-0109]
- National Institute on Minority Health and Health Disparities [G12MD007591] Funding Source: NIH RePORTER
Background: Candida albicans is a major opportunistic fungal pathogen. One of the most important virulence factors that contribute to the pathogenesis of candidiasis is its ability to form biofilms. A key characteristic of Candida biofilms is their resistance to antifungal agents. Due to significant morbidity and mortality rates related to biofilm-associated drug resistance, there is an urgency to develop novel nanotechnology-based approaches preventing biofilm-related infections. Methods: In this study, we report, for the first time, the synthesis of selenium nanoparticles by irradiating selenium pellets by nanosecond pulsed laser ablation in liquid chitosan as a capping agent. Synergy of the fungicidal effect of selenium nanoparticles and chitosan was quantified by the combination index theorem of Chou-Talalay. Results: This drug combination resulted in a potent fungicidal effect against a preformed C. albicans biofilm in a dose-response manner. By advanced electron microscopy techniques, we documented the adhesive and permeabilizing properties of chitosan, therefore allowing selenium nanoparticles to enter as the cell wall of the yeast became disrupted and distorted. Most importantly, we demonstrated a potent quantitative synergistic effect when compounds such as selenium and chitosan are combined. Conclusion: These chitosan-stabilized selenium nanoparticles could be used for ex vivo applications such as sterilizers for surfaces and biomedical devices.
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