期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 19, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/ijms19071969
关键词
Chlorella vulgaris; native promoter; photosystemI protein D (psaD); heterologous expression
资金
- Korea CCS R&D Center (KCRC) - Government of South Korea (Ministry of Science, ICT and Future Planning) [NRF-2014M1A8A1049273]
- Basic Core Technology Development Program for the Oceans and the Polar Regions of the National Research Foundation of Korea (NRF) - Ministry of Science, ICT [2015M1A5A1037053]
Chlorella has great potential as a bio-factory for production of value-added compounds. To produce the desired chemicals more efficiently in Chlorella, genetic tools for modification of Chlorella need to be developed, especially an endogenous promoter. In this study, the promoter of photosystem I protein D (psaD) from Chlorella vulgaris UTEX395 was identified. Computational analysis revealed the presence of several putative cis-acting elements, including a potential core element, and light-responsive or stress-responsive elements. Gene expression analysis in heterologous expression system in (C)hlamydomonas reinhardtii and Nicotiana benthamiana showed that CvpsaD promoter can be used to drive the expression of genes. Functional analysis of this promoter suggested that the initiator element (Inr) is important for its function (i.e., TATA-less promoter) and that an additional factor (e.g., downstream of the transcriptional start site) might be needed for light response. We have shown that the CvpsaD promoter is functional, but not sufficiently strong, both in microalgae and higher plant.
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