期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 19, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/ijms19040982
关键词
alpha v beta 3 integrin; extracellular matrix; fibronectin; Ehlers-Danlos syndromes; arterial tortuosity syndrome; apoptosis; fibroblast-to-myofibroblast transition
资金
- EDS patients' association C.E.D.I. (Clinici Ehlers-Danlos Italia) Onlus
- Fazzo Cusan family
- Italian Ministry of University (MIUR, Fondo per gli investimenti della ricerca di base)
The alpha v beta 3 integrin, an endothelial cells' receptor-binding fibronectin (FN) in the extracellular matrix (ECM) of blood vessels, regulates ECM remodeling during migration, invasion, angiogenesis, wound healing and inflammation, and is also involved in the epithelial mesenchymal transition. In vitro-grown human control fibroblasts organize a fibrillar network of FN, which is preferentially bound on the entire cell surface to its canonical alpha 5 beta 1 integrin receptor, whereas the alpha v beta 3 integrin is present only in rare patches in focal contacts. We report on the preferential recruitment of the alpha v beta 3 integrin, due to the lack of FN-ECM and its canonical integrin receptor, in dermal fibroblasts from Ehlers-Danlos syndromes (EDS) and arterial tortuosity syndrome (ATS), which are rare multisystem connective tissue disorders. We review our previous findings that unraveled different biological mechanisms elicited by the alpha v beta 3 integrin in fibroblasts derived from patients affected with classical (cEDS), vascular (vEDS), hypermobile EDS (hEDS), hypermobility spectrum disorders (HSD), and ATS. In cEDS and vEDS, respectively, due to defective type V and type III collagens, alpha v beta 3 rescues patients' fibroblasts from anoikis through a paxillin-p60Src-mediated cross-talk with the EGF receptor. In hEDS and HSD, without a defined molecular basis, the alpha v beta 3 integrin transduces to the ILK-Snail1-axis inducing a fibroblast-to-myofibroblast-transition. In ATS cells, the deficiency of the dehydroascorbic acid transporter GLUT10 leads to redox imbalance, ECM disarray together with the activation of a non-canonical alpha v beta 3 integrin-TGFBRII signaling, involving p125FAK/p60Src/p38MAPK. The characterization of these different biological functions triggered by alpha v beta 3 provides insights into the multifaced nature of this integrin, at least in cultured dermal fibroblasts, offering future perspectives for research in this field.
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