期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 19, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/ijms19020471
关键词
Ganoderma tsugae; laccase; targeted gene replacement; over-expression; development
资金
- Natural Science Foundation of Fujian Province for Distinguished Young Scholars [2014J06010]
- major platform for the development of edible fungus industry technology in Fujian Province [2014N21010008]
- Wu Xin Project of Agriculture of Fujian Provincial Development and Reform Commission of China [[2012]931]
- Research Funds for Distinguished Young Scientists in Fujian Agriculture and Forestry University [xjq201209]
- Spark program by Department of Science and Technology of Fujian Province [2016S0041]
In this study, a novel laccase gene (Lcc1) from Ganoderma tsugae was isolated and its functions were characterized in detail. The results showed that Lcc1 has the highest expression activity during mycelium development and fruit body maturation based on the analysis of Lcc1 RNA transcripts at different developmental stages of G. tsugae. To investigate the exact contribution of Lcc1 to mycelium and fruit body development in G. tsugae, Lcc1 transgenic strains were constructed by targeted gene replacement and over-expression approaches. The results showed that the lignin degradation rate in Lcc1 deletion mutant was much lower than the degradation efficiency of the wild-type (WT), over-expression and rescue strains. The lignin degradation activity of G. tsugae is dependent on Lcc1 and the deletion of Lcc1 exerted detrimental influences on the development of mycelium branch. Furthermore, the study uncovered that Lcc1 deletion mutants generated much shorter pale grey fruit bodies, suggesting that Lcc1 contributes directly to pigmentation and stipe elongation during fruit body development in G. tsugae. The information obtained in this study provides a novel and mechanistic insight into the specific role of Lcc1 during growth and development of G. tsugae.
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