期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 19, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/ijms19051404
关键词
adipose-derived stem cells; superficial adipose tissue; deep adipose tissue; immune cell infiltration; macrophages
Human abdominal subcutaneous adipose tissue consists of two individual layersthe superficial adipose tissue (SAT) and deep adipose tissue (DAT)separated by the Scarpa's fascia. The present study focuses on the analysis of morphological and immunological differences of primary adipocytes, adipose-derived stem cells (ASC), and tissue-infiltrating immune cells found in SAT and DAT. Adipocytes and stromal vascular fraction (SVF) cells were isolated from human SAT and DAT specimens and phenotypically characterized by in vitro assays. Ex vivo analysis of infiltrating immune cells was performed by flow cytometry. Primary adipocytes from SAT are larger in size but did not significantly differ in cytokine levels of LEPTIN, ADIPOQ, RBP4, CHEMERIN, DEFB1, VISFATIN, MCP1, or MSCF. ASC isolated from SAT proliferated faster and exhibited a higher differentiation potential than those isolated from DAT. Flow cytometry analysis indicated no specific differences in the relative numbers of ASC, epithelial progenitor cells (EPC), or CD3(+) T-cells, but showed higher numbers of tissue-infiltrating macrophages in SAT compared to DAT. Our findings suggest that ASC isolated from SAT have a higher regenerative potential than DAT-ASC. Moreover, spatial proximity to skin microbiota might promote macrophage infiltration in SAT.
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