期刊
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
卷 53, 期 8, 页码 1932-1941出版社
WILEY
DOI: 10.1111/ijfs.13780
关键词
Acylated monomeric anthocyanins; NMR; purple sweet potato; structural identification; UPLC-QTOF-MS/MS
资金
- Fundamental Research Funds for the Central Universities of China [2013PY095]
- Clinical Research Project of Health and Family Planning Commission of Wuhan Municipality [WX13A05]
- Research Project of Wuhan City Central Hospital [YQ15A04]
In recent years, researches on the isolation and preparation of monomeric anthocyanins have intensified because of the requirements of quantitative and structure-bioactivity relationship analyses. However, simple and effective methods about the scale of monomeric anthocyanins from the natural purple sweet potato powder are rarely reported. In this study, high molecular weight acylated monomeric anthocyanins were isolated from purple sweet potato (Ipomoea batatas L. cultivar Eshu No. 8) via the combination of column chromatography and semi-preparative HPLC technology and identified mainly by ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry/mass spectrometry (UPLC-QTOF-MS/MS) and H-1 and C-13 nuclear magnetic resonance (NMR). Two major acylated anthocyanins were unambiguously determined as peonidin 3-O-(6-O-(E)-caffeoyl-(2-O-(6-O-p-hydroxybenzoyl)--D-glucopyranosyl)--D-glucopyranoside)-5-O-(-D-glucopyranoside) and peonidin 3-O-(6-O-(E)-caffeoyl-(2-O-(6-O-(E)-feruloyl)--D- glucopyranosyl)--D-glucopyranoside)-5-O-(-D-glucopyranoside). The results of this study may help promote the purification of high molecular weight acylated anthocyanins from purple sweet potato as well as from other plant materials in nature.
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