期刊
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
卷 53, 期 9, 页码 2027-2036出版社
WILEY
DOI: 10.1111/ijfs.13783
关键词
Absorption characteristics; antioxidant activity; Dendrobium aphyllum; immunomodulatory effects; peptide fraction
资金
- Province Science and Technology of Guangdong [2016A040402020, 2016B010121014]
- Shaoguan City Science and Technology Project [2014CX/N310]
DA-P, fraction of peptides with a molecular weight <1kDa isolated from Dendrobium aphyllum, was analysed in three types of cell lines to verify its bioactivity and absorptivity. The cellular antioxidant activity of DA-P in HepG2 cells was used and results revealed an EC50 of 2.88 +/- 0.143mgmL(-1) and a CAA unit of 63.46 +/- 2.11m QE/100g peptides. DA-P treatment enhanced the secretion of cytokines in RAW 264.7 cells. After demonstrating the presence of tight junctions in Caco-2 monolayers, the absorption was 25.57%+/- 0.016% and 19.7%+/- 0.012% from different sides. The relatively high absorption indicated that the antioxidant-relevant immune functions of DA-P had a greater possibility to be absorbed by Caco-2 cells. Free amino acids and LC-MS/MS analysis indicated the degradation and expulsion of components after the absorption of DA-P, and Ser-Ser-Arg was able to come across the monolayers.
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