期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 266, 期 -, 页码 251-256出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2017.12.011
关键词
Digital PCR; Real-time PCR; Salmonella typhimurium; Limit of detection; Inhibitors
资金
- National Natural Science Foundation of China [31471202]
- Shandong Provincial Key Research and Development Program [2016YYSP009]
- Taishan Scholars Program of Shandong Province
As a kind of zero-tolerance foodborne pathogens, Salmonella typhimurium poses a great threat to quality of food products and public health. Hence, rapid and efficient approaches to identify Salmonella typhimurium are urgently needed. Combined with PCR and fluorescence technique, real-time PCR (qPCR) and digital PCR (ddPCR) are regarded as suitable tools for detecting foodborne pathogens. To compare the effect between qPCR and ddPCR in detecting Salmonella typhimurium, a series of nucleic acid, pure strain culture and spiking milk samples were applied and the resistance to inhibitors referred in this article as well. Compared with qPCR, ddPCR exhibited more sensitive (10(-4) ng/mu l or 10(2) cfu/ml) and less pre-culturing time (saving 2 h). Moreover, ddPCR had stronger resistance to inhibitors than qPCR, yet absolute quantification hardly performed when target's concentration over 1 ng/mu l or 10(6) cfu/ml. This study provides an alternative strategy in detecting foodborne Salmonella typhimurium.
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