4.7 Article

Comparison between digital PCR and real-time PCR in detection of Salmonella typhimurium in milk

期刊

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 266, 期 -, 页码 251-256

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2017.12.011

关键词

Digital PCR; Real-time PCR; Salmonella typhimurium; Limit of detection; Inhibitors

资金

  1. National Natural Science Foundation of China [31471202]
  2. Shandong Provincial Key Research and Development Program [2016YYSP009]
  3. Taishan Scholars Program of Shandong Province

向作者/读者索取更多资源

As a kind of zero-tolerance foodborne pathogens, Salmonella typhimurium poses a great threat to quality of food products and public health. Hence, rapid and efficient approaches to identify Salmonella typhimurium are urgently needed. Combined with PCR and fluorescence technique, real-time PCR (qPCR) and digital PCR (ddPCR) are regarded as suitable tools for detecting foodborne pathogens. To compare the effect between qPCR and ddPCR in detecting Salmonella typhimurium, a series of nucleic acid, pure strain culture and spiking milk samples were applied and the resistance to inhibitors referred in this article as well. Compared with qPCR, ddPCR exhibited more sensitive (10(-4) ng/mu l or 10(2) cfu/ml) and less pre-culturing time (saving 2 h). Moreover, ddPCR had stronger resistance to inhibitors than qPCR, yet absolute quantification hardly performed when target's concentration over 1 ng/mu l or 10(6) cfu/ml. This study provides an alternative strategy in detecting foodborne Salmonella typhimurium.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据