期刊
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
卷 272, 期 -, 页码 1-11出版社
ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2018.02.016
关键词
Fungi; Mycotoxins; Modelling; Inoculum size
资金
- Agenda de Gestio d'Ajuts Universitaris i de Recerca de la Generalitat de Catalunya (AGAUR) [2014FI_B 00045]
- Spanish Ministry of Economy and Competitiveness (MINECO) [AGL2014-55379-P]
Intraspecies variability in fungal growth and mycotoxin production has important implications for food safety. Using the Sioscreen C we have examined spectrophotometrically intraspecies variability of A. flavus using 10 isolates under different environments, including temperature shifts, in terms of growth and aflatoxin B-1 (AFB(1)) production. Five high and five low AFB(1) producers were examined. The study was conducted at 5 isothermal conditions (from 15 to 37 degrees C) and 4 dynamic scenarios (between 15 and 30 degrees C). The experiments were carried out in a semisolid YES medium at 0.92 a(w) and two inoculum levels, 10(2) and 10(3) spores/mL. The Time to Detection (TTD) of growth initiation was determined and modelled as a function of temperature through a polynomial equation and the model was used to predict TTD under temperature upshifts conditions using a novel approach. The results obtained in this study have shown that a model can be developed to describe the effect of temperature upshifts on the TTD for all the studied isolates and inoculum levels. Isolate variability increased as the growth conditions became more stressful and with a lower inoculum level. Inoculum level affected the in-traspedes variability but not the repeatability of the experiments. In dynamic conditions, isolate responses depended both on the temperature shift and, predominantly, the final temperature level. AFB(1) production was highly variable among the isolates and greatly depended on temperature (optimum temperature at 30-35 degrees C) and inoculum levels, with often higher production with lower inoculum. This suggests that, from an ecological point of view, the potential isolate variability and interaction with dynamic conditions should be taken into account in developing strategies to control growth and predicting mycotoxin risks by mycotoxigenic fungi.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据