期刊
GENE EXPRESSION
卷 16, 期 3, 页码 145-153出版社
COGNIZANT COMMUNICATION CORP
DOI: 10.3727/105221615X14181438356337
关键词
Chemotherapy-induced neuropathic pain; Paclitaxel; Anterior cingulate cortex (ACC); gamma-Aminobutyric acid (GABA); GABA transporter; GABA receptor
资金
- Kuwait University Research Sector [PT01/09, SRUL02/13]
The supraspinal pathophysiology of the painful neuropathy induced by paclitaxel, a chemotherapeutic agent, is not well understood. The gamma-aminobutyric acid (GABA) neurotransmitter system has been implicated in the pathogenesis of neuropathic pain. Gene expression of GABAergic system molecules was examined in the anterior cingulate cortex (ACC) of mice brains, by real-time PCR, during paclitaxel-induced neuropathic pain, because this area is involved in pain perception and modulation that might contribute to neuropathic pain. Paclitaxel treatment resulted in thermal hyperalgesia and in increased GABA transporter-1 (GAT-1) mRNA expression, but not that of other GABA transporters or GABAergic enzymes in the ACC compared to vehicle treatment. Among the 18 GABA(A) receptor subunits analyzed, only beta 2, beta 3, delta, and gamma 2 had increased mRNA levels, and for the GABA(B) receptor subunit, only GABA(B2) had increased mRNA levels in the ACC of paclitaxel-treated mice, whereas the rest of the GABA receptor subunits were not altered. The mRNA expression of GABA(A) receptor subunits alpha 6, theta, pi, rho 1, rho 2, and rho 3 were not detected in the ACC. In conclusion, these data show that during paclitaxel-induced neuropathic pain there is significant increase in GAT-1 expression in the ACC. GAT-1 is the main transporter of GABA from the synapse, and thus its increased expression possibly results in less GABA at the synapse and dysregulation of the GABAergic system. GAT-1 is a potential therapeutic target for managing paclitaxel-induced neuropathic pain.
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