期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
卷 14, 期 9, 页码 1099-1108出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.26400
关键词
ZIKV; envelope protein; G2/M arrest; mitotic catastrophe; apoptosis
资金
- National Key RD Projects [2017YFD0500600]
- Industrial High-tech Fields of Science and Technology Plan Projects of Guangdong Province [2013B010404026]
- Science and Technology Planning Project of Guangdong Province [2010A011200003, 2012B011000004, 2016A030303008, 2012B060300003]
- International SAMP
- T Cooperation Program of China [2011DFA33290]
- Medical Scientific Research Foundation of Guangdong Province [A2017124]
- Department of Education of Guangdong Province [2016KCXTD005, 2017KSYS010]
- Jiangmen Introduced Innovative Scientific Research Team Program
Clinical evidence suggests that there exists a strong correlation between Zika virus (ZIKV) infection and abnormal development of the nervous system. However, the underlying mechanisms remain to be elusive. In this study, recombinant lentiviral vectors coding for ZIKV structural proteins and truncations (prM-Env, M-Env and Env) were transduced into PC12 cells. Envelope (Env) overexpression induced significant inhibition of proliferation and triggered G2/M cell cycle arrest and apoptosis in PC12 cells. Flow cytometry and western blot analysis showed that the apoptosis was associated with up-regulation of both p53 and p21(Cip1/Waf1) and down-regulation of cyclin B1. Presence of aberrant nuclei clusters were confirmed by immunofluorescence staining analysis. The data indicate that overexpression of prM-Env, M-Env or Env led to apoptosis via an intrinsic cell death signaling pathway that is dependent on the activation of caspase-9 and caspase-3 and accompanied by an increased ratio of Bax to Bcl-2 in transduced PC12 cells. In summary, our results suggest that ZIKV Env protein causes apoptosis in PC12 cells via an intrinsic cell death signaling pathway, which may contribute to ZIKV-induced abnormal development of the nervous system.
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