期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 119, 期 -, 页码 524-532出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.07.137
关键词
Crude oil biodegradation; DNA and enzyme activity; Co-biodegradation
资金
- Shandong Provincial Natural Science Foundation, China [ZR2018MD017]
- Fundamental Research Funds for the Central Universities [201822009]
- National Key Research and Development Program [2016YFC1402301]
- Open Foundation of Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology of SOA [201702]
- Program for Innovative Research Team in University [IRT1289]
In this study, a cloned almA gene was inserted into two plasmids PUC57 and pet-28a (+), and then transformed into Escherichia coli competent strains. The presence of almA was confirmed by PCR combined with restriction enzyme digestion. The results indicated two strains of PUC57/almA-E. coli and pet-28a (+)/almA-E. coli were all functioned on biodegradation long-chain alkanes, however, pet-28a (+)/almA-E. coli strain performed better and the speed of biodegradation was enhanced to about 12 mg L-1 h(-1) when compared with PUC57/almA-E. coli strain. Furthermore, degradation degree of n-C32 was much higher than other long-chain alkanes for two engineered E. coli strains. Our results further suggested that exoenzyme was only detected in pet-28a (+)/almA-E. coli and followed a decreasing order of extracellular enzyme > intracellular enzyme > periplasmic enzyme. Besides, co-biodegradation of petroleum hydrocarbons by Pseudomonas sp. B-1, Bacillus subtilis PH-1 with exoenzyme or pet-28a (+)/almA-E. coli posed enormous potential that could reach 70%-80% in oil pollution bioremediation. (C) 2018 Elsevier B.V. All rights reserved.
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