期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 118, 期 -, 页码 1021-1034出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.06.183
关键词
Folate bioreceptor; Cell targeting; Cytosensor; Nitrogen doped graphene quantum dots; Cancer diagnosis
资金
- Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
- Liver and Gastrointestinal Diseases Research Center of Tabriz University of Medical Science under Ethical Committee License of the National Institute for Medical Research Development [IR.NIMAD.REC.1396210]
In recent years, study of folate receptor (FR) expression related to targeting, drug delivery and counting of tumoral cells have been followed. In this work, a fast and simple strategy was reported to determine the FR expressed cancer cells based on the selective bonding of the folic acid/folate (FA) to the FR-positive tumor cells. The folate decorated Nitrogen-doped graphene quantum dots (N-GQDs) were utilized as selective targeting of the MKN 45 cells. Fluorescent microscopy imaging investigations revealed that the produced FA conjugated NGQDs could specifically attach to the target FR-positive tumor cells. Due to the fluorescence emission of N-GQDs, the developed cytosensor is free from attaching any fluorescent ligand i.e. Rhodamine B to capture the florescence microscopy images and also flow cytometry analysis. The fabricated cytosensor possesses a dynamic range from 100 to 7.0 x 10(4)cell.mL(-1) with high selectivity. Furthermore, the cytosensor also could visualized the MCF 7 and HT 29 cells where the dynamic ranges were 100 to 1.0 x 10(4) and 500 to 4.0 x 10(4) cells.mL(-1), respectively. In vitro toxicity tests has shown low toxicity of the synthesized N-GQDs where the minimum viability is 68%. The proposed FA-N-GQDs based cytosensor provides a novel platform for detection of MKN 45, HT 29 and MCF 7 cancer cell lines which could be used in multi-channel cancer diagnosis biodevice. (C) 2018 Published by Elsevier B.V.
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