In vitro and in vivo anti-inflammatory activities of high molecular weight sulfated polysaccharide; containing fucose separated from Sargassum horneri: Short communication

Recent studies on crude and pure compounds from Sargassum horneri have shown promising bioactive properties. However, anti-inflammatory potentials of fucose-rich sulfated polysaccharides from S. horneri have hot yet been discovered. In the present study, we evaluated the in vitro and in vivo anti-inflammatory activities of four polysaccharide fractions separated from membrane filters according to their molecular weights (<5 kDa (f1), 5-10 kDa (f2), 10-30 kDa (f3), and >30 kDa (f4)), According to the results, F4 fraction inhibited the lipopolysacch arides (LPS)-induced nitric oxide (NO) (IC50 = 87.12 mu g/mL) and prostaglandin E-2 production as well as pro-inflammatory cytokine production in RAW 264.7 cells through down-regulating nuclear factor-kappa B signaling cascade. According to the results, f4 has a potential to down-regulate LPS-induced toxicity, cell death and NO production levels in LPS-induced in vivo zebrafish embryo model. These results suggest that f4 fraction has the potential to develop functional materials or drugs to treat inflammatory diseases. (C) 2017 Elsevier B.V. All rights reserved.