期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 107, 期 -, 页码 35-41出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.08.148
关键词
Jambo type II arabinogalactan; THP-1 cells; Cytokine secrection
资金
- Projeto Universal [477971/2012-1]
- CAPES [140745/2013-0]
- CNPq foundation (Brazil) [140745/2013-0]
- PRONEX-Carboidratos
Syzygium jambos is an Indo-Malaian found in many tropical countries and it is mainly composed of carbohydrates. Fraction PF-WSP and SF-WSP were obtained by aqueous extraction followed by Fehling's treatment. Monosaccharide analysis showed that fraction PF-WSP has a high content of uronic acids (90%) and fraction SF-WSP presented mainly galactose (39.1%) and arabinose (34.2%), as neutral sugars and 9% of galacturonic acid. The presence of type II arabinogalactan in SF-WSP was evidenced by methylation analysis and C-13/H-1 HSQC NMR experiments. Immunomodulating properties of SF-WSP was investigated. It decreases THP-1 macrophage viability at the highest concentration tested (200 mu g/mL). We then tested non-cytotoxic concentrations of SF-WSP on THP-1 cytokine production in the presence and absence of LPS. The results showed that SF-WSP increased TNF-alpha, IL-1 beta and IL-10 secretion in a concentration-dependent manner as well as attenuated the inflammatory response induced by LPS at the highest concentration (100 mu g/mL). These results contribute to elucidate the effects of fruit pectic polysaccharides on immune cells. (C) 2017 Elsevier B.V. All rights reserved.
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