期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 112, 期 -, 页码 976-984出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.01.196
关键词
Antioxidant activity; Drying procedures; Polysaccharide; Spent Lentinus edodes substrate
资金
- Key Technologies R&D Program of Tianjin [14ZCZDNC00008]
- Tianjin Transformation and Promotion Program of Agricultural Science and Technology Achievement [201701230]
- National Key Research and Development Program [2017YFD0201400]
- New Century outstanding talent support program, Education Ministry of China
- China Scholarship Council
This study explored the potential of spent Lentinus edodes substrate, a by-product of mushroom industries causing environmental pollution, serving as materials to produce antioxidant polysaccharide. The extraction process of spent Lentinus edodes substrate polysaccharide (SLSP) was optimized and the effects of drying methods on chemical composition, morphological property and antioxidant activity were investigated. Results showed that freeze-dried SLSP (SLSP-F) exhibited the best quality in terms of the polysaccharide yield (13.00%) and antioxidant activity. The EC50 values of SLSP-F on DPPH, ABTS and superoxide anion radicals was 0.051 mg/mL, 0379 mg/mL, 0.719 mg/ml, respectively, which was significantly lower than that of freeze-dried Lentinus edodes polysaccharide (LP-F). After purification by Sephadex G-150, the purified SLSP-F (PSP) has a molecular weight of 16.77 kDa. Compared with LP-F, PSP has more reducing sugars and uronic acids in chemical composition and higher contents of xylose, glucose and galactose in monosaccharide composition. FT-IR and NMR spectroscopy analysis revealed that PSP has both alpha and beta glycosidic bonds and massive acetyl groups, which is different from LP-F mainly composed of 1, 3 linked alpha-D-Manp residue with some acetyl groups. The findings provided a reliable approach for the development of antioxidant polysaccharide from spent Lentinus edodes substrate. (C) 2018 Elsevier B.V. All rights reserved.
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