期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 116, 期 -, 页码 502-512出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.05.002
关键词
Enterocin-B; Heterodimer of enterocin-A plus B; Cloning and overexpression; Anti-biofilm activity; Anti-cancer activity
资金
- UGC, New Delhi, India
- DBT-IPLS-Pondicherry University [BT/PR14554/INF/125/2010]
In this present study, a gene (ent-B) encoding the bacteriocin enterocin-B was cloned, overexpressed and purified from Enterococcus faecium port. The molecular weight of the bacteriocin enterocin-B was observed around 7.2 kDa and exhibited antimicrobial activity against several human pathogenic bacteria. The antimicrobial activity of cloned enterocin-B was increased effectively by combining with another bacteriocin enterocin-A from the same microorganism. Protein-protein docking and molecular dynamics simulation studies revealed that the bacteriocin enterocin-B is interacting with enterocin-A and formation of a heterodimer (enterocin A + B). The heterodimer of bacteriocin enterocin-A + B exhibited potential anti-bacterial, anti-biofilm activity against Staphylococcus aureus, Acinetobacter baumannii, Listeria monocytogenes and Escherichia coli. The bacteriocin enterocin-B, A and heterodimer of bacteriocin enterocin A + B showed no haemolysis on human RBC cells. This is the first report that the cell growth inhibitory activity of the bacteriocin enterocin B against HeLa, HT-29 and AGS human cancer cells and this cell growth inhibitory activity was significantly increased when cancer cells treated with the heterodimer of bacteriocins enterocin-A + B. The cell growth inhibitory activity of the bacteriocin enterocin-B and the heterodimer of bacteriocin enterocin-A + B were not observed in non-cancerous INT-407 cells (intestinal epithelial cells). (C) 2018 Published by Elsevier B.V.
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