期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 116, 期 -, 页码 831-839出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.01.165
关键词
Fructus aurantii; Polysaccharides; Structural characterization; Immune modulator; Pro-IL-1
资金
- National Natural Science Foundation of China [81603366]
- Natural Science Foundation of Heilongjiang [H2015041]
A purified polysaccharide, designated CALB-4, was acquired from Fructus aurantii that is the traditional edible/medicina plant in China. The present study was performed to characterize the CALB-4 and to evaluate its immunomodulatory activities on human peripheral blood mononuclear cells (PBMCs). The structure of CALB-4 was characterized by partial add hydrolysis, periodate oxidation, Smith degradation, and methylation analysis combined with gas chromatography-mass spectrometry (GC-MS), Infrared Spectroscopy (IR) and scanning electron microscopy (SEM). The results indicated that CALB-4 was elucidated as a pectic polysaccharide and its main chain is composed of Man, Gal UA and Gal, interspersed with Ara, Rha, Man and Gal. Furthermore, immunological tests showed that CALB-4 exhibits the immunoenhancement effects. The mechanism for this action might be attributed to the increase of the cytoplasmic concentration of pro-IL-1 via the up-regulation of several mitogen-activated protein kinases (MAPKs) and the nuclear translocation of p65. This study clarified that CALB-4 could be as an efficacious biological response modifier in immunotherapy. (C) 2018 Published by Elsevier B.V.
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