期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 113, 期 -, 页码 583-591出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2018.02.131
关键词
Pinecone polyphenols of Pinus koraiensis; Microspheres preparation; Optimization; Sustained-release characteristics; Radiation protection
资金
- NSFC [31170510]
- Thirteen five national key research and development projects [2016YFC0500305-02]
- scientific research innovation fund of Harbin Institute of Technology [HIT.NSRIF.2017023]
Here, the chitosan and the glutaraldehyde (GA) were used to encapsulate pinecones polyphenols of Pinus koraiensis (P. koraiensis) by emulsification cross-linking technology. First, the prepared parameters (crosslinking agent amount, stirring speed, crosslinking temperature and emulsifying time) of the pinecones polyphenols microspheres (PPMs) were optimized by the response surface methodology (RSM). When chitosan concentration and crosslinking time were 2% and 80 min, respectively, the optimal conditions were 7.91 mL of crosslinking agent, stirring speed of 660.98 r/min, crosslinking temperature of 41.18 degrees C and emulsifying time of 198.65 min. The prepared PPMs embedding rate was 73.57%. The optimized PPM possessed a distinct core-shell structure and uniform spherical distribution with a particle size value of 3.4 mu m. In addition, they had the excellent sustained-release characteristics in vitro. We also evaluated the radioprotective effects of PPMs against Co-60-gamma radiation in vivo. PPMs improved significantly the activity of the antioxidant enzyme SOD and reduce MDA level in the plasma of irradiated mice. Accordingly, PPMs could also significantly enhance the immunomodulation activity by promoting the proliferation of splenocytes and monocyte phagocytosis of irradiated mice. These results suggested that PPMs exert effective protection against radiation-induced injury by improving the antioxidant and immunomodulation activities. (C) 2018 Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据