期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 110, 期 -, 页码 497-503出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.10.105
关键词
Cell printing; Cell-laden structure; Collagen; Cross-linking; Tannic acid
资金
- National Research Foundation of Korea grant - Ministry of Education, Science, and Technology (MEST) [NRF-2015R1A2A1A15055305]
- Korea Healthcare Technology RAMP
- D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea [HI15C3000]
- Korea Health Promotion Institute [HI15C3000020016] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2018H1A2A1063241] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Cell-printing is an emerging technique that enables to build a customized structure using biomaterials and living cells for various biomedical applications. In many biomaterials, alginate has been widely used for rapid gelation, low cost, and relatively high processability. However, biocompatibilities enhancing cell adhesion and proliferation were limited, so that, to overcome this problem, an outstanding alternative, collagen, has been extensively investigated. Many factors remain to be proven for cell-printing applications, such as printability, physical sustainability after printing, and applicability of in vitro cell culture. This study proposes a cell-laden collagen scaffold fabricated via cell-printing and tannic acid (TA) crosslinking process. The effects of the crosslinking agent (0-3 wt% TA) in the cell-laden collagen scaffolds on physical properties and cellular activities using preosteoblasts (MC3T3-E1) were presented. Compared to the cell-laden collagen scaffold without TA crosslinking, the scaffold with TA crosslinking was significantly enhanced in mechanical properties, while reasonable cellular activities were observed. Concisely, this study introduces the possibility of a cell-printing process using collagen and TA crosslinking and in vitro cell culture for tissue regeneration. (C) 2017 Elsevier B.V. All rights reserved.
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