期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 117, 期 -, 页码 1280-1288出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2018.06.001
关键词
Protein quantum dot interaction; Effect of ZnO QDs on global transcription regulator; CRP (Cyclic AMP Receptor Protein); Modulation of protein structure and function; Transcription inhibition; Determination of binding site
资金
- FNP through Homing fellowship [Homing/2017-3/22]
- CSIR
ZnO quantum dots (QDs) are very well known for their antimicrobial activity against several bacteria, however, we still do not know any protein targets of ZnO QDs. In order to determine possible protein target, interaction of ZnO QDs was studied with CRP (Cyclic AMP Receptor Protein), a global transcription regulator protein. Binding between ZnO QDs and E. coli CRP was mainly studied by isothermal titration calorimetry (ITC), structural changes of protein were monitored by fluorescence and circular dichroism spectroscopy, and in-vitro transcription assay was used to asses CRP activity. Result shows that both electrostatic and hydrophobic interactions are involved in CRP-ZnO binding. Different spectroscopic investigation revealed that ZnO binding to CRP leads to extensive unfolding and destabilization, which ultimately leads to protein aggregation. It was also observed that in presence of ZnO dimerization ability of CRP was sharply reduced. In-vitro transcription assay also shows that CRP activity gets severely compromised on ZnO binding. All our data suggests that ZnO QD binding to CRP and consequent structural and functional changes most probably plays a crucial role in ZnO (20 induced antimicrobial action. (C) 2018 Elsevier B.V. All rights reserved.
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