期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 115, 期 -, 页码 281-286出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2018.04.083
关键词
Oxidative stress; Osteoarthritis; Antioxidant; Angelica sinensis polysaccharide
资金
- Priority Specialty Fund of Jiangsu Province
This study aimed to investigate the oxidative stress in human osteoarthritis (OA) chondrocytes and the antioxidant effect of angelica sinensis polysaccharide (ASP) on it. Human OA chondrocytes and human normal chondrocytes induced by hydrogen peroxide (H2O2) alone or treated with ASP were cultured, then they were measured with cell viability, levels of reactive oxygen species (ROS), nitric oxide (NO) and malondialdehyde (MDA) production, activities of inducible nitric oxide synthase (iNOS), superoxide dismutase (SOD) and catalase (CAT), expression of related genes, such as collagen type II (Col2a1), Aggrecan, iNOS, SOD, CAT and peroxisome proliferator-activated receptor gamma (PPAR gamma). We found that the production of ROS, NO, and MDA and the activity of iNOS, SOD and CAT were significantly increased in OA chondrocytes compared with human normal chondrocytes. When treated with H2O2, human chondrocytes exhibited acute oxidative stress injure, which was similar to OA chondrocytes. Pretreatment with ASP before H2O2 alleviated oxidative stress and protected human chondrocytes. The activity of PPAR gamma was decreased in OA chondrocytes and increased when treated with ASP. In conclusion, oxidative stress is an important pathogenesis of OA, and ASP inhibits H2O2-mediated injury in human chondrocytes. Our data also suggest that PPAR gamma participates in oxidation and anti-oxidation of OA (C) 2018 Elsevier B.V. All rights reserved.
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