期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 114, 期 -, 页码 324-333出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.03.116
关键词
Natural polymers; Cryogels; Chitosan; 1,1,3,3-tetramethoxypropane
资金
- European Union's Horizon 2020 Research and Innovation Programme under the Marie Skiodowska-Curie Fellowship grant [701289]
The chitosan and gelatine are commonly used biopolymers for the tissue engineering applications. In the previous methods for the cryogels synthesis, multistep preparation methods using toxic cross-linking agents such as glutaraldehyde are reported. Here, we present a two-step preparation method of gelatin macroporous cryogels and one-step preparation method of chitosan or gelatin cryogels. The physico-chemical properties of obtained scaffolds were characterized using FTIR, zeta potential, SEM and laser confocal microscopy. Non-toxic and biodegradable cross-linking agents such as oxidized dextran and 1,1,33-tetramethoxypropane are utilized. The one-step chitosan cryogels had degradation degree-2 times higher compared to the cryogels prepared with a two-step method i.e. reduced by borohydride. Scaffolds cross-linked by glutaraldehyde had about 40% viability, whereas nine various compositions of cryogels showed significantly higher viability (similar to 80%) of fibroblast cells in vitro. The cryogels were obtained without using the harmful compounds and therefore can be used straightforward as biocompatible and biodegradable scaffolds for the cell culturing purposes and other biomedical applications. (C) 2018 Published by Elsevier B.V.
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