期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 112, 期 -, 页码 473-482出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2018.02.003
关键词
Rosa roxburghii; Polysaccharide; Microwave-assisted enzymatic extraction
资金
- Scientific Research Foundation of Development and Reform Commission of Jilin province, China [2014N149]
- Jilin Scientific and Technological Development Program [20160209013YY]
An extraction assay applying microwave-assisted enzymatic treatment for polysaccharides in Rosa roxburghii was developed using response surface methodology. The process parameters were optimized using Plackett-Burman (PB) design and central composite design to enhance the Rosa roxburghii polysaccharide extraction yield. Specific conditions (microwave power, 575 W; microwave time, 18 min; liquid-to-material ratio, 13.5:1 mL/g; and enzyme dose, 6.5 g/mL) generated an experimental yield of 36.21 +/- 0.62%, which closely agreed with the predicted value of 35.75%. Purification with a DEAE-52 cellulose column generated two fractions, PR-1 (from 6.2 x 10(3) to 7.4 KDa) and PR-2 (from 559.8 to 106.6 KDa). Subsequently, the antioxidant activity and alpha-D-glucosidase inhibitory activity of the two polysaccharide fractions were assessed; PR-1 exhibited stronger antioxidant activity and alpha-D-glucosidase inhibitory activity than PR-2. Finally, the monosaccharide composition of PR-1 was determined by HPLC using a 1-phenyl-3-methyl-5-pyrazolone precolumn derivatization method. The result showed that PR-1 contained mannose, ribose, rhamnose, glucosamine hydrochloride, glucuronic acid, galacturonic acid, glucose, galactose, arabinose and fucose with molar percentages of 2.1%, 0.54%, 2.1%, 0.26%, 1.5%, 22.7%, 24.0%, 26.4%, 19.6% and 0.89%, respectively. (C) 2018 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据