Coproduction of protease and mannanase from Bacillus nealsonii PN-11 in solid state fermentation and their combined application as detergent additives

Bacillus nealsonii PN-11 produces thermo-alkalistable mannanase and protease active in wide temperature and pH range. Optimization of coproduction of protease and mannanase from this strain and application of cocktail of these enzymes as detergent additives were studied. On optimization mannanase yield of 834 Ug(-1) (11.12 fold increase) and protease yield of 70 Ug(-1) (4.7 fold increase) could be obtained in a single fermentation. Purification and characterization of mannanase have been done earlier and protease was done during this study and has a molecular mass of 48 kDa. pH and temperature optima for protease were 10.0 and 65 degrees C respectively. It was completely stable at 60 degrees C for 3 h and retained >80% of activity at pH 11.0 for 1 h. Both the enzymes were compatible with detergents individually and in a combination. The wash performance of the detergent on different type of stains improved when protease or mannanase were used individually. However destaining was more efficient when a combination of mannanase and protease was used. (C) 2017 Elsevier B.V. All rights reserved.